Authors:
Title:
Caracterización del cultivo miceliar de Laccaria bicolor (Maire) P. D. Orton (Agaricales) en medio MMN con diferentes pH. --
Classification:
RCQF20101801j
Summary:
Caracteriza el crecimiento miceliar in vitro de una cepa nativa de Laccaria bicolor (196.1997), recolectada en Huehuetenango, Guatemala, en medio Melin-Norkrans Modificado (MMN) con 4 niveles de pH: 4.5, 5.5, 6.5 y 7.0, incubando las placas durante 40 días a 26ºC. Determinó que el mayor diámetro de las colonias se obtuvo en el pH 7.0. La morfología de las colonias varió según los tratamientos: con pH 4.5 y 5.5 la cepa produjo acúmulos hifales, mientras que con pH 6.5 y 7.0 las colonias fueron lisas y fibrilosas. Microscópicamente se observaron hias de 2.0-4.0 um, con hinchamientos irregulares en los septos, a modo de nódulos. --
ABSTRACT: A Guatemalan strain of Laccaria bicolor (L. bicolor 197.1997), collected in Huehuetenango, was characterized in vitro to determine its miceliar growth under four different pH conditions: 4.5, 5.5, 6.5 and 7.0 in Modified Melin-Norkrans Medium (MMN). The plates were incubeted at 26ºC and studied for 40 days. The biggest diameter was obtained in pH 7.0 and variation in the morphology of the colonies was observed according to the pH level: with pH 4.5 and 5.5 hyphal tufts were formed while fibrillous and smooth colonies were observed in 6.5 and 7.0 pH cultures. The hyphae showed a 2.0-4.0 um in diameter with irregular swollen cells, as nodules, at the septa and a notorious violet pigmentation in the acid pH cultures. --
ABSTRACT: A Guatemalan strain of Laccaria bicolor (L. bicolor 197.1997), collected in Huehuetenango, was characterized in vitro to determine its miceliar growth under four different pH conditions: 4.5, 5.5, 6.5 and 7.0 in Modified Melin-Norkrans Medium (MMN). The plates were incubeted at 26ºC and studied for 40 days. The biggest diameter was obtained in pH 7.0 and variation in the morphology of the colonies was observed according to the pH level: with pH 4.5 and 5.5 hyphal tufts were formed while fibrillous and smooth colonies were observed in 6.5 and 7.0 pH cultures. The hyphae showed a 2.0-4.0 um in diameter with irregular swollen cells, as nodules, at the septa and a notorious violet pigmentation in the acid pH cultures. --
Original document:
En: Revista Científica de la Facultad de Ciencias Químicas y Farmacia y del Instituto de Investigaciones Químicas y Biológicas de la USAC ; (Vol.18, No.1) p. 89-94-- Guatemala : USAC, 2010. 2070-8246.
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